Production of alginates with high viscosifying power and molecular weight by using the <scp>AT9</scp> strain of <i>Azotobacter vinelandii</i> in batch cultures under different oxygen transfer conditions

Background Alginate is a polysaccharide constituted by β-d-mannuronic acid and α-l-guluronic which has several applications in the field of tissue engineering, drug delivery other biomedical applications. This study aimed to evaluate production, molecular weight (MW) viscosifying power alginate produced strain AT9 cultured 3-L reactor, under three different oxygen transfer rates (OTR). Results An increase OTRmax, range 3.6–12.4 mmol L−1 h−1 promoted cellular growth production cultures, reaching specific rate 0.14 maximal concentration 2.9 g cultures developed at 12.4 h−1. By contrast, low OTR (3.6 h−1) 2927 kDa was obtained high OTRmax (12.4 acetylation degree (2–3%) reached. As result higher alginate, highest (3.16 L g−1). Conclusion Overall, our results indicate that controlling it possible manipulate MW, bacterial alginates AT9. © 2022 The Authors. Journal Chemical Technology Biotechnology published John Wiley & Sons Ltd on behalf Society Industry (SCI).